Figure S5.

Characterization of the differentiation process of BA differentiated with medium 2 and analysis of pro-inflammatory signaling cascades. (A) Representative western blotting. The graphs show UCP-1 protein levels (n = 5 independent experiments) and Ucp1 mRNA levels relative to day 0 (n = 6 independent experiments). P.S., Ponceau staining. (B) Measurement of the nitrite concentration in CM from Raw 267.4 macrophages (n = 4 independent experiments). (C) Representative western blotting. The graphs show the fold increase in phosphorylation levels vs CM-CTR in BA-WT and BA-SIRT1^Tg+ (n = 3–5 independent experiments). Bars represent mean ± SEM. In panels A and B, the statistical analysis was conducted with Mann–Whitney U test. ^∗Comparison between genotypes or treatments. In panel C, the statistical analysis was performed with two-way ANOVA. ^∗Comparison with CM-LPS at 30 min in the same genotype. ^#Comparison between genotypes with the same treatment. ^∗,#p < 0.05, ^∗∗p < 0.01, and ^∗∗∗p < 0.001.