Figure S7.

Levels of acetyl-p65 NFκB and effect of differentiation of BA with T₃, insulin, and rosiglitazone-enriched medium on ATP levels under normal or pro-inflammatory conditions. (A) Representative western blotting of acetyl p65-NFκB in differentiated BA with medium 1 or 2 (n = 2 independent experiments). (B) The graph shows ATP levels under conditions described in A (n = 4–6 independent experiments). (C) Graphs show ATP levels in differentiated BA with the two media after stimulation with CM-CTR or CM-LPS (n = 4–6 independent experiments). Note the panels' different scales. Bars represent mean ± SEM. Statistical analysis was performed with two-way ANOVA. In panel B, ∗comparison between media in the same genotype. ^#Comparison between genotypes with the same medium. In panel C, ^∗comparison between LPS-treated or untreated cells in the same medium. ^#Comparison between media with the same treatment. ^∗∗p < 0.01 and ^{∗∗∗,###}p < 0.001. P.S., Ponceau staining.