Figure 2.

Moderate SIRT1 overexpression ameliorated the effects of LPS in BAT. (A) Western blotting of SIRT1 in BAT and quantification (n = 4 mice/group). (B) Western blotting analysis and quantification of STAT3, JNK, and p38 MAPK phosphorylation in BAT of the WT and SIRT1^Tg+ mice intraperitoneally (i.p.) injected with LPS (2 mg/kg) and sacrificed at different time periods (n = 2–5 mice/group). (C)Il6 and Tnfa mRNA expression in BAT (n = 4 mice/group). (D) Western blotting and quantification showing levels of insulin-induced IR and AKT (Ser 473) phosphorylation after LPS and insulin injections (n = 5–6 mice/group). (E) Western blotting and quantification of UCP-1 protein expression in BAT from the mice receiving LPS or not and exposed to 4 °C for the last 6 h (n = 8–10 mice/group). The right panel shows UCP-1 immunohistochemistry images (n = 3 mice/group). (F) The graphs show skin temperature of the BAT area and rectal temperature (n = 7–17 mice/group). The middle panel shows representative images. (G) Nuclei quantification in different BAT sections (3–4 mice/group). The right panel shows representative H&E staining BAT images. Scale bars represent 50 μm. Data are expressed as mean ± SEM. Statistical analysis in A, D, and E was conducted with the Mann–Whitney U test. Statistical analysis in B, C, F, and G was performed by two-way ANOVA. ^∗Comparisons between LPS-treated and untreated mice with the same genotype. ^#Comparison between genotypes receiving the same treatment. ^∗,#p < 0.05, ^∗∗,##p < 0.01, and ^∗∗∗p < 0.001.