Figure 3.

Light microscopy images of the cells. Untreated cell lines in culture and RB-treated cells after 48 h treatment with an IC₅₀ concentration of RB. Bright field images are viewed under low power (4× objective). Treated melanoma cells (WM164, WM1366, and D24) appeared rounded, shrunken, and damaged. Many of the dead cells were detached from the plate and floating in the media. HaCaT cells only showed some morphological changes but remained attached to the surface and Fibroblast cells remained intact. Scale bar = 200 µm.