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. 2020 Oct 11;12(10):1151. doi: 10.3390/v12101151

Figure 2.

Figure 2

Summary of utilized adenoviruses (AdV) and schematic outline of DNA sequences contained in the AdV genomes. The upper panel provides a schematic overview of contained DNA sequences in the AdV genomes and the lower panel summarizes AdV types used for this study and respective species. The GFP, luciferase, neomycin (GLN)-reporter cassette encoding turboGFP (tGFP), nanoLuciferase (nLuc), and neomycin (neo) was inserted into the E3 region in the opposite orientation of major later promoter (MLP) of the adenovirus genome. ITR: inverted terminal repeat; Ψ: packaging signal; E1A, E1B, E2A, E2B, E3, E4: early adenovirus transcription units; L1-L4: late adenovirus genes, T2A and P2A: self-cleaving peptides; CAG: promoter with cytomegalovirus early enhancer element, part of the chicken beta-actin promoter and splice acceptor of the rabbit beta-globin gene; pA: polyadenylation signal. Blue horizontal arrows: early gene transcription units, blue dotted arrows: late gene transcription units, vertical red arrow: schematically presenting cleavage peptides. The figure was adapted from Zhang et al. [19].