Figure 5.

Experimental setup to analyze the effect of AdV and the house dust mite (HDM) on NAEPCs. During the nasal brushing procedure, we collected the cells in cell culture medium, centrifuged at 350× g for 8 min, and washed with PBS. The cell pellet was resuspended in cell culture medium, and the cells were seeded in collagen I pre-coated T75 flasks. After passage 2 (P2) was reached, NAEPCs were collected and seeded in collagen I pre-coated 96 well plates. Different HDM concentrations were used (1 µL/mL, 10 µg/mL, 100 µg/mL). The transduction concentration of AdV5 was set at 10 virus particle per cell (vpc). This figure was generated using BioRender.com