Figure 6.

The effect of HDM stimulation and AdV5 infection on NAEPCs. NAEPCs were stimulated at different time points (day 1 and day 3) with different HDM concentrations (1 µg/mL, 10 µg/mL, and 100 µg/mL), and the cells were subsequently transduced with AdV5 at 10 virus particle numbers per cell (vpc). Twenty-four hours post-transduction, luciferase assays were performed. We observed an increased AdV5 transduction efficiency in pre-stimulated NAEPCs with HDM, particularly at day 3 in asthmatic specimens. This was in contrast to samples of healthy controls. Values were normalized and presented as fold change given as mean and standard error of mean (SEM).