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. 2020 Sep 25;12(10):615. doi: 10.3390/toxins12100615

Figure 5.

Figure 5

Expression of cytokine (IFN-γ, IL-17, IL-2, and TNF-α) mRNA in the blood leukocytes after non-specific stimulation with PMA and ionomycin. The data were obtained from the piglets after intrauterine DON exposure (DON in grey columns) and compared to the control piglets (Con in white columns) at 1, 3, and 18 weeks after birth. Data acquired by quantitative real-time PCR are expressed as the mean (columns) of multiples of the reference gene expression (HPRT units) and the standard deviation (error bars). The number of piglets in each group was as follows: 1 week control, n = 36; 1 week DON, n = 39; 3 weeks control, n = 33; 3 weeks DON, n = 38; 18 weeks control, n = 27; 18 weeks DON, n = 32. The Mann–Whitney test was used for statistical analysis. Significant differences between the DON and control groups are marked as * p < 0.05, ** p < 0.01, or *** p < 0.001.