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. 2020 Oct 9;8(10):403. doi: 10.3390/biomedicines8100403

Figure 5.

Figure 5

Polyinosinic-polycytidylic acid (poly I:C) act as a substitute of stearic acid cooperative effect on MIP-1α/CCL3 production. (A,B) THP-1 cells were treated (via transfection) with poly I:C (5 µg) for 2 h and then incubated with BSA (control) or stearic acid or TNF-α for 24 h. MIP-1α/CCL3 mRNA and protein were determined. The results obtained from minimum three independent experiments with three replicates of each experiment are shown. All data are expressed as mean ± SEM (n ≥ 3); * P < 0.05; ** P < 0.01, when compared with stearic acid or TNF-α alone. (C,D) Western blot analysis showed that individuals with obesity have significant higher levels of phospho-IRF3 in monocytes compared to lean individuals (P = 0.0179).