Table 1.
LC-MS/MS parameters for the quantitative analysis of 22 phenolic compound standards.
| Compounds | Retention Time | Q1 (m/z)a | Q3(m/z)b | DP (V)c | EP (V)d | CEP (V)e | CE (eV)f | CXP (V)g |
|---|---|---|---|---|---|---|---|---|
| L-Phenylalanine | 5.80 | 163.888 | 146.8 | −41.000 | −10.500 | −12.000 | −18.000 | −10.000 |
| Gallic acid | 5.92 | 169.056 | 125.000 | −26.000 | −10.000 | −8.000 | −20.000 | −6.000 |
| Homogentisic acid | 7.10 | 166.920 | 122.900 | −21.000 | −7.000 | −14.000 | −14.000 | −28.000 |
| Protocatechuic acid | 8.04 | 152.896 | 108.900 | −16.000 | −9.000 | −10.000 | −22.000 | −6.000 |
| Chlorogenic acid | 8.30 | 352.846 | 191.000 | −21.000 | −7.000 | −32.000 | −30.000 | −10.000 |
| Orientin | 9.69 | 447.092 | 327.000 | −61.000 | −11.000 | −20.000 | −22.000 | −54.000 |
| Rutin | 9.98 | 609.002 | 299.700 | −91.000 | −10.500 | −34.000 | −52.000 | −14.000 |
| p-Hydroxybenzoic acid | 10.02 | 136.885 | 92.900 | −16.000 | −8.500 | −12.000 | −24.000 | −6.000 |
| Caffeic acid | 10.10 | 178.868 | 134.800 | −16.000 | −8.000 | −14.000 | −22.000 | −6.000 |
| Vitexin | 10.36 | 430.826 | 310.800 | −56.000 | −9.000 | −28.000 | −22.000 | −50.000 |
| Vanillic acid | 10.46 | 166.883 | 151.900 | −11.000 | −6.500 | −10.000 | −18.000 | −32.000 |
| Gentisic acid | 10.63 | 152.871 | 107.900 | −16.000 | −8.500 | −6.000 | −28.000 | −22.000 |
| 2,4-Dihydroxybenzoic acid | 11.42 | 152.900 | 108.900 | −21.000 | −4.500 | −10.000 | −18.000 | −6.000 |
| p-Coumaric acid | 12.08 | 162.866 | 118.900 | −11.000 | −7.000 | −14.000 | −20.000 | −6.000 |
| Ferulic acid | 12.44 | 192.881 | 133.900 | −11.000 | −10.000 | −12.000 | −22.000 | −8.000 |
| m-Coumaric acid | 12.58 | 162.886 | 118.900 | −21.000 | −9.000 | −8.000 | −22.000 | −4.000 |
| Veratric acid | 12.96 | 180.991 | 136.900 | −16.000 | −7.500 | −12.000 | −18.000 | −8.000 |
| Luteolin | 15.77 | 284.966 | 133.200 | −66.000 | −10.000 | −16.000 | −46.000 | −30.000 |
| Quercetin | 16.11 | 300.884 | 150.800 | −31.000 | −10.500 | −18.000 | −28.000 | −32.000 |
| Salicylic aci | 16.58 | 136.874 | 92.900 | −16.000 | −7.500 | −12.000 | −22.000 | −6.000 |
| Apigenin | 18.50 | 268.948 | 117.000 | −46.000 | −10.000 | −20.000 | −56.000 | −8.000 |
a Precursor ion (Q1, m/z); b fragment ion (Q3, m/z); c DP: declustering potential; d EP: entrance potential; e CE: collision energy; f CEP: cell entrance potential; g CXP: collision cell exit potential.