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Apoptotic study. (A) Annexin V-FITC/PI apoptosis assay in A549 and A549/T cells after treatment with CM-HNPs at a fixed PTX concentration of 5 µg/mL for 24 h at 37 °C. (B) Cellular and nuclear morphology of cells after treatment. Scale bar = 100 µm. (C) Caspase-3 activity of A549/T cells after treatment with CM-HNPs for 24 h at 37 °C; n = 3, one-way ANOVA and Tukey analysis applied, and level of significance was *** p < 0.001 vs. blank.
