Figure 11.
P-gp drug efflux inhibition. (A) Fluorescence images of A549 and A549/T cells after 1, 2, and 4 h incubation with the free Rhodamine 123 (RH-123) (5 mM) in the absence and presence of A549/T CM-HNPs and A549/T CM-PTX-NPs at a fixed PTX concentration of 5 µg/mL at 37 °C, scale bar: 100 µm. (B) Fluorescence intensity of A549/T cells after 1, 2, and 4 h incubation with the free RH-123 (5 mM) in the absence and presence of A549/T CM-HNPs and A549/T CM-PTX-NPs at a fixed PTX concentration of 5 µg/mL at 37 °C. (C) Intracellular ATP concentration in A549/T cells at 4 h post-treatment. * p < 0.05, ** p < 0.01, and *** p < 0.001 vs. free RH-123 and control, respectively.