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. 2020 Oct 7;10(10):1981. doi: 10.3390/nano10101981

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Biophysical characterization of the self-assembly of M2e-I₁₀ into fibrils. (a) Determination of critical aggregation concentration (CAC) by plotting the pyrene I₃₇₃/I₃₈₄ fluorescence ratio vs. M2e-I₁₀ concentration. (b) Absorbance measurement over time of self-assembly of M2e-I₁₀. Raw data and non-linear Boltzmann fit are presented. (c,d) Kinetics of self-assembly of M2e-I₁₀ measured by far-UV circular dichroism (CD) spectroscopy and (d) ThT fluorescence, with excitation at 440 nm. (e) Representative TEM image of M2e-I₁₀ assembled for 168 h (left panel) and diffraction pattern obtained by powder X-ray diffraction (PXRD) (right panel).