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M2e-NFs display high density of the M2e epitope at the surface. (a) Measurement of zeta potential indicating the surface charge of M2e-NFs and of non-functionalized fibrils (NFs). (b) ELISA analysis of M2e peptide, NFs, monomeric M2e-I10, and M2e-NFs. (c) TEM images of NFs and M2e-NFs after immunogold staining with anti-M2 primary antibody and gold-conjugated secondary antibody.
