Figure 5.

Comparative electrophoretic mobilities of 5 µM control duplex FAM-17′/17 (Lanes 1–4), dodecyl-containing duplex FAM-17′/D-17^PG (Lanes 5–8) and the FAM-D-17^PG conjugate (Lanes 9–12) investigated by EMSA in non-denaturing 8% PAAG after 2 h incubation at 37 °C in DMEM medium (depicted in red), DMEM supplemented with 30 µM BSA (depicted in green), DMEM supplemented with 10% fetal bovine serum (FBS) (depicted in blue). All medium conditions without oligomers control lanes are depicted on the right in corresponding colors. Indicated oligomers after incubation in PBS are depicted in black for additional controls. Bands were visualized after electrophoresis by Stains-All staining (a) and by recording the image after scanning with laser excitation at 488 nm (b). It is interesting to note that during the electrophoretic analysis of micellar assemblies of FAM-labeled DOCs, we observed significant fluorescence quenching of their bands, in contrast with the bands of non-conjugated control oligomers (Figure 5, Lanes 9, 12, Figure S20).