Figure 7.

Cellular uptake of fluorescently labelled DOC (FAM-D-17^PG) and its duplex (D-17^PG/FAM-17′) estimated by flow cytometry after 4 h incubation of HepG2 cells with conjugates and additional followed 14 h without it: (a) percentage of FAM-positive HepG2 cells in the population; (b) normalized median value of the cell fluorescence to the autofluorescence of control cells. The highest transfection efficacy was achieved for cells treated with FAM-D-17^PG in DMEM (Figure 7a,b). All cells were transfected (Figure 7a), and the median value of their fluorescence intensity exceeded that of cells’ auto-fluorescence 11 ± 1 times (Figure 7b). High efficacy of transfection was also reached for cells transfected with duplex D-17^PG/FAM-17′ in DMEM (Figure 7a). Surprisingly, the median value of cells’ fluorescence was almost two times lower than for cells treated with a single stranded dodecyl-containing oligonucleotide (Figure 7b).