Figure 1.

Schematic showing the workflow for our modified deep sequence-coupled biopanning (DSCB) approach. IgG was isolated from human serum and mixed with an MS2 virus-like particle (VLP) library displaying all possible 10-amino acid peptides from the dengue virus (DENV) polypeptide. Protein-G coupled to magnetic beads are used to pull down antibody–VLP complexes. Bound VLPs are eluted, RNA is extracted, and RT-PCR is used to produce cDNA, which is then subjected to Ion Torrent deep sequencing.