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Fresh gonadal primordial germ cells (gPGCs) and circulating blood primordial germ cells (cPGCs) observed under an inverted phase-contrast microscope; gPGCs and cPGCs are visualized in darkfield illumination. The PGCs are distinguished as bright, illuminated cells among other cell types (P.434242 patent application number). Magnification with 40× objective (Leica DMI8, Germany).
