Figure 3.

Isoprene metabolic gene clusters. Isoprene metabolic gene clusters in isoprene-degrading isolates (including Sphingopyxis strain OPL5 and Gordonia strain OPL2 isolated in this study), and a representative contig with the iso gene cluster obtained from metagenome co-assembly of ¹³C-DNA from isoprene-enriched willow soil (wsMG4). The isoA gene that codes for the alpha-subunit of the monooxygenase is shown in red. The % identity of iso gene-encoded polypeptides to the corresponding Iso polypeptides of the well-characterized Rhodococcus strain AD45 are shown in Table S2. omp: outer membrane protein transfer.