Table 2.

Experimental design of Freund’s adjuvant-induced sub-acute inflammation.

Groups/Abbrev.	Administrated Substances/Dose/Time	Route
Group I—control group (CTRL)	Normal saline solution	p.o.
Group II—positive control group (CTRL+)	Diclofenac sodium (5 mg/kg BW)	i.p
Group III—(NSO_Prev)	NSO (4 mL/ kg BW)/ 7 days before FA	p.o.
Group IV—(NSO_Treat)	NSO (4 mL/ kg BW)/ 7 days after FA	p.o.
Group V—(NSO_Adj)	NSO (4 mL/ kg BW) + diclofenac sodium (5 mg/kg -BW) / 7 days after FA	p.o./ i.p

Abbreviations: BW, body weight; FA, Freund’s adjuvant; i.p, intraperitoneally; NSO, Nigella sativa oil; p.o, orally.