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. 2020 Oct 8;12(10):650. doi: 10.3390/toxins12100650

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Fragment patterns by multiplex PCR assays of representative reference strains of Clostridium perfringens. Multiplex PCR: 50 bp ladder are reported on the left. Lines 1–8: Clostridium perfringens strains: ATCC 13124 (type A), KF190/06 (type A-consensus CPB2), ATCC 12917 (type F old A-CPE), NCTC 6121 (type B), NCTC 3180 (type C), NCTC 8346 (type D), NTCT 8084 (type E). Line 9: Clostridium sordellii ATCC 9714 (negative control). Simplex PCRs Lane 10: FDP82/04 (type A-atypical CPB2), Lane 11: 50 bp ladder, Lane 12: strain LBV/18 (type G-netB), Lane13: 50 bp ladder.