. 2020 Oct 8;12(10):650. doi: 10.3390/toxins12100650

Table 4.

Oligonucleotide primers used in this study for Clostridium perfringens toxin genes detection.

Toxinotypes Detected	Target Gene	PCR	Primer	Sequence (5′--->3′)	AL	[Ref.]
A, B, C, D, E, F, G	cpa	M	CPA-F	GTTGATAGCGCAGGACATGTTAAG	402	[56]
A, B, C, D, E, F, G	cpa	M	CPA-R	CATGTAGTCATCTGTTCCAGCATC	402	[56]
B, C	cpb	M	CPB-F	ACTATACAGACAGATCATTCAACC	236	[56]
B, C	cpb	M	CPB-R	TTAGGAGCAGTTAGAACTACAGAC	236	[56]
B, D	etx	M	CPETX-F	ACTGCAACTACTACTCATACTGTG	541	[56]
B, D	etx	M	CPETX-R	CTGGTGCCTTAATAGAAAGACTCC	541	[56]
E	iap	M	CPITX-F	GCGATGAAAAGCCTACACCACTAC	317	[56]
E	iap	M	CPITX-R	GGTATATCCTCCACGCATATAGTC	317	[56]
F (C, D, E)	cpe	M	CPE-F	GGGGAACCCTCAGTAGTTTCA	506	[57]
F (C, D, E)	cpe	M	CPE-R	ACCAGCTGGATTTGAGTTTAATG	506	[57]
(A, B, C, D, E, F, G)	cons-cpb2	M	CPB2CON-F	CAAGCAATTGGGGGAGTTTA	200	[57]
(A, B, C, D, E, F, G)	cons-cpb2	M	CPB2CON-R	GCAGAATCAGGATTTTGACCA	200	[57]
(A, B, C, D, E, F, G)	aty-cpb2	S	CPB2ATY1-25F	AGGAATTCACAAAATGAATACAGTTAAAGCAAATG	750	[26]
(A, B, C, D, E, F, G)	aty-cpb2	S	CPB2ATY1-25R	GTGATGATGACCGGTATAACAATAACCCTC	750	[26]
G	netB	S	JRP6656	CTTCTAGTGATACCGCTTCAC	738	[4]
G	netB	S	JRP6655	CGTTATATTCACTTGTTGACGAAAG	738	[4]

M: Multiplex PCR set up and optimized for this study; S: Simplex PCR; AL: amplicon length (bp); cons-cpb2 = consensus cpb2; aty-cpb2 = atypical cpb2; () in these toxinotypes toxin genes targeted can be optionally present.