Table 2.
Primers used in this study.
| Gene | Primers | Sequence (5′–3′) | Usage |
|---|---|---|---|
| PaPP5 | PaPP5D-F | CACATGTCCCTGGGCAAGTWYAARYTNGC | Degenerate primers |
| PaPP5D-R | GGNAARTGYATCACCGTGTTCTCCGCC | Degenerate primers | |
| PaPP5 | 5PaPP5-1 | TGGAACTTCTTGTGGGCGA | 5′ RACE |
| 5PaPP5-2 | GAATAATATCCACGGTCAACA | 5′ RACE | |
| 3PaPP5-1 | GAAGGTGAAGTAAAGGCGAAG | 3′ RACE | |
| 3PaPP5-2 | GAAATAGGCAACCTCCAGAAG | 3′ RACE | |
| PaPP5c | PaPP5c-F | CCCATATGCACCACCACCACCACCACTACAGCGGACCCAAGCTTG Nde1 |
Amplification of PaPP5c |
| PaPP5c | PaPP5c-R | CCCTCGAGTCACATCATTCCTAGCTGC Xho1 |
Amplification of PaPP5c |