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. 2020 Oct 11;12(10):3089. doi: 10.3390/nu12103089

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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RJ-related fatty acids increase myoblast differentiation in C2C12 cells. (A–C) C2C12 cells were treated with myogenic medium along with 500 μM 10H2DA, 10HDAA, 2DA, or SA for 6 days. Cells were then stained with anti-myosin heavy chain antibody (A). Fusion index was quantified as the number of nuclei within myotubes (at least three) divided by the total number of nuclei (B). Western blotting showing protein levels of myosin heavy chain (Myhc), Myogenin, or Gapdh determined (C). Representative images are shown. Scale = 100 μm (A and C). Data are mean ± SD (n = 4). ∗∗p < 0.01, versus DMSO treatment.