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. 2020 Oct 11;12(10):2923. doi: 10.3390/cancers12102923

Figure 3.

Figure 3

UM-EVs promote proliferation, migration and invasion of BRCA1-deficient fibroblast (Fibro-BKO) cells. (A–C) Fibro-BKO cells were cultured for 3 weeks in the presence of culture medium without EVs (No-EVs), NCM-EVs (from PPWc and PPxG eye donors) or UM-EVs. (A,B) Cells were analyzed for their growth potential by measuring population doubling capability at every passage. Data in inserts represent cumulative population doublings at the end of the treatment periods (A). Column graphs represent pooled data from three EV-free medium (No-EVs) samples, two NCM-EVs preparations and five UM-EVs preparations. Data are mean ± SD. p values ˂ 0.05 (*) (B). (C–F) Fibro-BKO cells were cultured for 12 h (C,D; cell migration) or 24 h (E,F; cell invasion) in the presence of culture medium without EVs (PBS), NCM-EVs or UM-EVs. Data are mean  ±  SD. (D) p value = 0.0086 (**), p value = 0.0051 (#). (F) p value = 0.0072 (**), p value < 0.0001 (****), MEL270 UM-EVs/OMM2.5 UM-EVs, p value = 0.0033 (**) (n = 3). Scale bar:100 µm, magnification 100×.