Figure 4.

In vivo tumorigenicity assay of Fibro-BKO cells treated with UM-EVs (A)Exposed cells were injected subcutaneously into NOD/SCID mice that were monitored for 4 weeks for tumors growth. At euthanasia, developing tumors were excised and their sizes were measured. (B) Formalin-fixed paraffin-embedded tumors were processed for H&E staining, and immunolabeled with anti-Ki67, anti-Vimentin and anti-MelanA antibodies. Scale bar: 10 µm. Red arrowheads pointed to mitotic figures, and black arrow pointed to a melanophage. Positive controls are from choroidal melanoma specimens.