Figure 5.

The gp5Cβ–gp5.4 complex interacts with spheroplast PpiD. The purified complex was incubated with spheroplasts in the presence of 0.5% formaldehyde for 10 min (Lanes 1 and 3). After affinity purification, the complex coeluted with periplasmic protein PpiD, which was identified by matrix-assisted laser-desorption/-ionization (MALDI) analysis (Lane 1, asterisk). As control, the affinity-purified gp5Cβ–gp5.4 complex is shown (Lane 2). To break the cross-link, sample was heated at 100 °C (lane 3).