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. 2020 Oct 8;10(10):1428. doi: 10.3390/biom10101428

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Hypothesized pathways to produce C₁₈ FA in A. minutum. Numbers in boxes correspond to the final AE value (white for neutral lipids and black for polar lipids). The triangles symbolize the desaturases (front-end in yellow and methyl-end in purple), the circles the enzymes involved in the PKS pathway (KS: 3-ketoacyl synthase; KR: 3-ketoacyl-ACP-reductase; DH: dehydrase; 2.2I: 2-trans, 2-cis isomerase; 2.3I: 2-trans, 3-cis isomerase; ER: enoyl reductase). The ways with dashed arrows appear to be unlikely or cannot be proven with the enrichment dynamics. NL and PL written on the routes allowing the synthesis of 18:5n-3 indicate the fraction considered for each pathway.