Table 2.
Cellular parameters of A. minutum: morphology Side Scatter (SSC) and Forward Scatter (FSC)), viability (FL1-SYTOX), chlorophyll content (FL3), and neutral lipid content (FL1-BODIPY) using flow cytometry analysis (mean ± SD). Values for SYTOX are in % of total cells, and the values for BODIPY/FL3/SSC/FSC are in arbitrary units (a.u).
| (h) | FL1-SYTOX ALIVE |
FL1-BODIPY | FL3 | SSC | FSC | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 0 | 100 | ± | 0 | 107 | ± | 18 | 547 | ± | 5 | 627 | ± | 13 | 164 | ± | 5 |
| 2.5 | 100 | ± | 0 | 115 | ± | 11 | 549 | ± | 17 | 639 | ± | 8 | 161 | ± | 5 |
| 5 | 99 | ± | 1 | 118 | ± | 3 | 574 | ± | 25 | 639 | ± | 7 | 162 | ± | 0 |
| 10 | 100 | ± | 0 | 158 | ± | 25 | 599 | ± | 9 | 676 | ± | 0 | 164 | ± | 1 |
| 20 | 100 | ± | 0 | 130 | ± | 22 | 601 | ± | 15 | 653 | ± | 15 | 141 | ± | 11 |
| 24 | 100 | ± | 0 | 90 | ± | 7 | 606 | ± | 12 | 730 | ± | 22 | 159 | ± | 7 |
| 30 | 100 | ± | 1 | 96 | ± | 10 | 534 | ± | 3 | 707 | ± | 26 | 167 | ± | 5 |
| 48 | 100 | ± | 0 | 58 | ± | 9 | 554 | ± | 14 | 697 | ± | 15 | 162 | ± | 1 |
| 54 | 100 | ± | 0 | 81 | ± | 3 | 522 | ± | 2 | 602 | ± | 6 | 181 | ± | 5 |