Continuous exposure of uninfected cells to antiviral doses of TG does not affect cell viability. Cell viability of (A) NPTr cells continuously incubated in the presence of 0.5 μM TG and (B) NHBE cells continuously incubated with 5 nM TG was monitored over 24 h with RealTime-Glo™ MT Cell Viability Assay. Readings indicate duration of TG exposure. Significance based on mixed effect analysis relative to corresponding DMSO control. Cell viability of (C) uninfected NPTr cells and (D) NPTr cells infected with 0.5 MOI USSR H1N1, pre-primed with 0.5 μM TG for 30 min, was monitored with RealTime-Glo™ MT Cell Viability Assay. Readings indicate duration of post mock or influenza virus infection. Significance based on mixed effect analysis relative to corresponding DMSO control. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.