Figure 6.

TG in mice confers protection against lethal PR8 H1N1 virus challenge. (A) Survival of mice post-infection treated each day with TG or PBS-DMSO control (n = 10 in each group). Kaplan-Meier survival curves are compared using the log-rank (Mantel-Cox) analysis. (B) Viral titres of lungs from mice treated with TG or PBS-DMSO at 3dpi and 5dpi was determined by TCID₅₀ assays (n = 3 in each group). (C) Mean body weight changes post-infection was determined by daily monitoring (n = 10 in each group). (D) At 5 dpi, entire lungs of TG treated mice displayed much less extensive gross pathology than those of control lungs. Blue outline demarcates boundary between apparent normal and consolidated abnormal tissues. (E) Representative microscopic lung fields, (40-time magnification), derived from similar anatomical sites taken at 3 and 5 dpi, showed that TG treatment resulted in less diffused distribution of viral NP protein (less brown staining), and more frequent localisation of NP than those in corresponding PBS-DMSO controls. Each mouse was infected at 1 × 10² TCID₅₀ of PR8 virus. ** p < 0.01, *** p < 0.001.