Figure 2.

Saccharomyces cerevisiae mt-aaRS predicted-MTS and its conservation. The hundred N terminal amino acids of each S. cerevisiae mitochondrial aaRS were aligned to their human, Mus musculus, Danio rerio, Schizosaccharomyces pombe (when available) orthologs [51]. Consensus sequence is indicated above aligned sequences. Also indicated are the positions of the S. cerevisiae amphipathic helix (continues boxes), Tom20 recognition sequence (dashed red boxes) and Mitochondrial Processing Protease (MPP) cleavage site (arrowheads), predicted by MitoFates [45]. Note that MTS sequence conservation is very low and conserved regions are usually functional domains downstream to the MPP. A higher resolution image is provided as a supplementary material (Supplementary Figure S1).