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. 2020 Oct 8;9(10):825. doi: 10.3390/pathogens9100825

Figure 2.

Figure 2

The immunosuppressive effect of LTA is TLR2-dependent in LPS-stimulated BMDCs. (A–C) BMDCs were pre-treated with anti-TLR2 mAb or isotype antibody at 37 °C for 1 h and then stimulated with LPS (100 ng/mL) combined with LTA (5 ug/mL) or vehicle control. The culture was further incubated at 37 °C for 24 h, then the TNF-α production was measured by ELISA (A). The expression of MyD88, pErk1/2, pp38 and pp65 was analyzed by flow cytometry (B, C). Data are shown as the representative and mean ± SEM of five samples in independent experiments. Student’s t-test was used to analyze data for significant differences. Values of * p < 0.05, ** p < 0.01 and *** p < 0.001 were regarded as significant.