Figure 4.
LTA suppresses antigen-specific effector CD4+ T cell generation. (A–C) BMDCs were cocultured with splenic CD4+ T cells isolated from OT-II mice in the presence of OVA peptide (OVA323–339: 10 ug/mL). Some cultures were further treated with LPS (100 ng/mL), LPS+LTA (5 μg/mL) or LTA. The samples treated with LTA only or vehicle were negative control (no-Ag). The cultures were incubated at 37 °C for 72 h. At the last 5 h, the cells were re-stimulated with Phorbol 12-myristate 13-acetate (PMA)/ionomycin, then IFN-γ+CD4+ T (Th1) cells (A, B) and CD69 expression on the population (C) were analyzed by flow cytometry. Data are shown as the representative and mean ± SEM of five samples in independent experiments. Student’s t-test was used to analyze data for significant differences. Values of * p < 0.05, ** p < 0.01 and *** p < 0.001 were regarded as significant.