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. 2020 Oct 8;12(10):1140. doi: 10.3390/v12101140

Figure 1.

Figure 1

Reactivity of human proteins to bNAbs in Surface plasmon resonance (SPR): EDC3 blocks interaction between CR6261 and recombinant H1-HA. (A) SPR assay was performed with the MAbs captured on a protein-G sensor chip followed by addition of recombinant host protein (20 µg/mL) selected using ProtoArray. (B) Sensorgrams depicting the reactivity of serial dilutions of recombinant EDC3 in SPR to determine the binding affinity of recombinant EDC3 to the CR6261 by SPR. (C) Sensorgram to show competition between EDC3 and HA0 binding to CR6261. The binding of recombinant H1–HA0 (10 µg/mL) to the CR6261 bound EDC3 (10 µg/mL) was measured by SPR. Total H1–HA0 binding to CR6261 in absence of any EDC3 binding was defined as 100%. Percentage inhibition by the EDC3 was determined. (D) Structural depiction of potential binding site of CR6261 contact residues on EDC3 (PDB #2vc8). The EDC3 potential binding residue numbers and residues shaded in yellow, cyan, and green shown are depicted in similar colors on the EDC3 structure.