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. 2020 Oct 13;10(10):156. doi: 10.3390/bs10100156

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Two experimental designs progressed in parallel: #1. Behavioral study; #2. Molecular biology study. Unilateral neurotoxic lesion of right pedunculopontine nucleus was performed by injection of N-methyl-D-aspartate (NMDA) (Sigma, St. Louis, MO, USA) solution at a rate of 0.1 µL/min using a 5 µL Hamilton syringe. Experimental design #1: Motor coordination was studied by means of Grid test 7 and 30 days after pedunculopontine nucleus injury. When the behavioral studies concluded, the rats were sacrificed for morphological studies. Experimental design #2: Nigral Nrf2 mRNA expression was studied 24 h, 48 h and 7 days after PPN injury. The selection of lesioned/sham-operated rats to form the 48-h and 7-days post injury group was completely randomized.