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. 2020 Oct 2;9(10):2230. doi: 10.3390/cells9102230

Figure 5.

Figure 5

Loss of TAZ favours neuronal differentiation. (A) schematic overview of the experimental procedure. ReNcells VM were transduced with lentivirus encoding shcontrol (shCO) or human shTAZ, and after five days were plated under differentiation conditions (in the absence of growth factors) for 30 days. Representative confocal immunofluorescence photographs of DCX, MAP2, and TAZ (B), TUBB3 (C), and TAU (D). Nuclei are counterstained with DAPI. Quantifications correspond to DCX+ (E), MAP2+ (F), TUBB3+ (G), and TAU+ (H) cells. (I) Dendrite length based on MAP2 staining; (J) axonal length based on TAU staining. Data are mean ± S.E.M (n = 20). Statistical analysis was performed with the Student’s t-test. * p < 0.05; ** p < 0.01, *** p < 0.005.