Figure 6.

TEADs participate in the inhibitory effect of TAZ on neuronal differentiation. (A) schematic overview of the experimental design. ReNcells VM were transduced with empty vector (CT) or retroviral vector for overexpression of TAZ-WT and TAZ mutants TAZ^S51A, TAZ^4SA and TAZ^4SA+S51A. After five days of retroviral transduction, cells were incubated under differentiation conditions for 30 days; (B) immunostaining with neuronal markers DCX, MAP2, and TAU. Nuclei are counterstained with DAPI. Quantification of DCX⁺ (C), MAP2⁺ (D), and TAU⁺ (E) cells. (F) dendrite length based on MAP2 staining; (G) axonal length based on TAU staining. Data are mean ± S.E.M. (n = 20). Statistical analysis was performed with one-way ANOVA. * p < 0.05; ** p < 0.01, *** p < 0.001 vs. CT. $$ p < 0.01, $$$ p < 0.001 comparing TAZ^S51A vs. TAZ-WT. ### p < 0.001 comparing TAZ^4SA+S51A vs. TAZ^4SA.