Figure 7.

TAZ/TEAD is a transcriptional repressor of SOX2 and proneuronal transcription factors. (A) representative immunoblots of TAZ, CTGF, SOX2, NEUROD1, and GAPDH as a loading control in ReNcells VM CT, TAZ-WT, TAZ^S51A, TAZ^4SA, and TAZ^4SA+S51A after five days of retroviral transduction; (B) densitometric quantification of SOX2 protein levels in (A) relative to GAPDH; (C) densitometric quantification of NEUROD1 protein levels in (A) relative to GAPDH. Data are mean ± SEM (n = 4). (D) mRNA levels of TAZ targets CTGF and CYR61. (E) mRNA levels of SOX2, ASCL1, NEUROG2 and NEUROD1. mRNA levels were determined by qRT-PCR and normalized by the geometric mean of ACTB, GAPDH and TBP levels. Data are presented as mean ± S.D. (n = 4). Statistical analysis was performed using one-way ANOVA. * p < 0.05; ** p < 0.01, *** p < 0.001 vs. control conditions. $$$ p < 0.001 comparing TAZ^S51A vs. TAZ-WT. # p < 0.05; ### p < 0.001 comparing TAZ^4SA+S51A vs. TAZ^4SA. (F) mRNA levels of TEAD1, TEAD2, TEAD3 and TEAD4 in ReNcells VM after 5 days of lentiviral transduction with control vector (shCO) or shRNA against co-expression of shRNAs for TEAD1/3/4, and TEAD2 (shTEAD). (G) mRNA levels of CTGF and CYR61; (H) mRNA levels of SOX2, ASCL1, NEUROG2, and NEUROD1. mRNA levels were determined by qRT-PCR and normalized by the geometric mean of ACTB, GAPDH and TBP levels. Data represent mean ± S.D. (n = 4). Statistical analysis was performed using one-way ANOVA. *** p < 0.001 vs. shCO.