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. 2020 Oct 9;12(10):2892. doi: 10.3390/cancers12102892

Figure 2.

Figure 2

Murine macrophages robustly internalize MSN. (a) Flow cytometry analysis of cell association with fluorescent MSNs following incubation with 10 µg/mL DyLight 488-conjugated-MSN for 1, 3, or 24 h at 37 °C (top graph). Percent of cells positive for fluorescent MSN association (middle graph) or mean fluorescent intensity (MFI; bottom graph) of cells 1 h after the addition of 10−100 µg/mL MSN. (b–e) Pseudo-colored scanning electron microscopy (SEM) images of RAW 264.7 cells 1 h after the addition of MSN (red) to the culture media. (b) Macrophage with a cluster of MSN (red) on the cell surface. (c) Cell filopodia with a bound MSN (pseudo-colored red) at the distal end. (d) MSN (red) on cell bodies and TNTs. (e) MSN (red) uptake by filopodia projecting from non-adherent cellular bridges (a.k.a. TNTs). * p < 0.05; ** p < 0.01.