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. 2020 Oct 9;12(10):2892. doi: 10.3390/cancers12102892

Figure 6.

Figure 6

MSN internalization without heterotypic nanoparticle transfer between syngeneic RAW 264.7 macrophages (Mac) and 4T1 cancer cells. (a) Flow cytometry analysis of fluorescent MSN uptake presented as percent positive cells and mean uptake per population. (b) Merged DIC and fluorescent image of a 4T1 cancer cell surrounded by adherent macrophages (green, phalloidin). (c) SEM images of co-cultured 4T1 cancer cells and RAW 264.7 macrophages (round cells) showing direct cellular contact. (d) Lack of heterotypic MSN transfer shown using 3D merged DIC and fluorescent images of 24 h co-cultured CellTracker Violet positive 4T1 cancer cells and MSN-loaded (red) macrophages (nuclei, blue, DAPI). * p < 0.05, ** p < 0.01.