Figure 8.

Association of MSN with macrophages in immune-competent syngeneic mice following intravenous injection. BALB/C mice with 4T1 breast tumors were injected intravenously with 250 µg DyLight 633-labeled MSN (red) directly (d, free) or via the adoptive transfer of splenocytes preloaded with MSN (a–c). Twenty-four-hour post-administration, filtering organs and tumors were excised and labeled with either F4/80 or CD204 fluorescent antibodies (green). (a) Liver and spleen with no antibody staining (green, autofluorescence). (b) F4/80-labeled (green) tissues. (c,d) CD204-labeled (green) tissues. Arrows highlight cell-to-cell connections with (white) or without (yellow) MSN.