Table 2.
Antibiofilm SCVA, anti-CSH and anti-QS activities of cBM MSC CM.
| Indices | cBM MSC CM Effects | ||
|---|---|---|---|
| Anti-Biofilm a (A550 ± SD) | Anti-CSH b (A570 ± SD) | Anti-QS c (RLU/A550 ± SD) | |
| PC | 0.391 ± 0.062 | 0.331 ± 0.022 (59%) | 11,714 ± 1362 |
| NC | 0.346 ± 0.099 | 0.349 ± 0.054 (56%) | 12,253 ± 332 |
| CM AM | 0.274 ± 0.133 * | 0.437 ± 0.032 (45%) *** | 7753 ± 700 *** |
| CM L | 0.197 ± 0.092 *** | 0.440 ± 0.025 (45%) *** | 10,294 ± 387 * |
| CM AST | 0.246 ± 0.055 * | 0.440 ± 0.052 (45%) *** | 7803 ± 162 *** |
| CM P | 0.150 ± 0.096 *** | 0.450 ± 0.040 (44%) *** | 9735 ± 547 *** |
| CM E | 0.215 ± 0.047 ** | 0.451 ± 0.053 (44%) *** | 10,230 ± 532 ** |
| CM B | 0.257 ± 0.183 ** | 0.556 ± 0.053 (31%) *** | 8920 ± 237 *** |
a average values of absorbance ± SD are presented, A550–absorbance at λ = 550 nm; SA 14–Staphylococcus aureus 14 treated and/or untreated with conditioned media (CM) from canine bone marrow mesenchymal stem cell (cBM MSC). b average values of absorbance after adherence to n-hexadecan from eight replicate measurements ± SD are presented, A570–absorbance at λ = 570 nm; SA 14 treated and/or untreated with canine bone marrow mesenchymal stem cell-conditioned media (cBM MSC CM). Optical density before adding n-hexadecane was adjusted to 0.8. The hydrophobicity was expressed as the percentage of bacterial cells adhering to n-hexadecan. c normalized bioluminescence [Relative Light Units (RLU/A550 ± SD)] after 24 h growth for the bioluminescence reporter E. coli pSB1142 exposed to P. aeruginosa CFCS producing long-chain AHLs (C10–C14) treated and/or untreated with cBM MSC CM. Alaskan Malamute (AM), Labrador (L), American Staffordshire Terrier (AST), Poodle (P), Eurohound €, Beagle (B). A one way ANOVA and Tukey’s post hoc test were used to detect significant differences between the positive control and individual experimental groups, and a statistical significance was accepted at * p value < 0.05, ** p value < 0.01 and *** p value < 0.001