Table A2.
Limit of Quantification (LoQ) and Limit of Detection (LoD) of S. cerevisiae (Sc), T. delbrueckii (Td), L. thermotolerans (Lt) and M. pulcherrima (Mp) obtained by PMAxx-qPCR. DNA from living cells of each species (Sc, Td, Lt or Mp) was mixed with DNA from dead cells of the same species (living/dead mixed cultures), or with DNA from living cells of another yeast species (living/living mixed cultures, Sc/Td, Td/Sc, Lt/Sc or Mp/Sc). Cycle threshold was calculated by triplicate and standard deviation was calculated (Ct ± SD).
| Living/Dead Mixed Cultures | |||
|---|---|---|---|
| Living Yeast (evaluated concentrations) | Dead Yeast | LoQ (Ct ± SD) | LoD (Ct ± SD) |
| Sc (104 to 10 CFU/mL) |
Sc (106 CFU/mL) | 103
(27.907 ± 0.124) |
102 (30.841 ± 0.468) |
| Td (104 to 10 CFU/mL) |
Td (106 CFU/mL) | 103 (28.670 ± 0.139) |
102 (30.817 ± 0.273) |
| Lt (104 to 10 CFU/mL) |
Lt (106 CFU/mL) | 103 (29.594 ± 0.180) |
102 (32.255 ± 0.483) |
| Mp (104 to 10 CFU/mL) |
Mp (106 CFU/mL) | 104 (28.570 ± 0.190) |
103 (32.030 ± 0.940) |
| Living/Living Mixed Cultures | |||
| Living Yeast (evaluated concentrations) | Living Yeast | LoQ (Ct ± SD) | LoD (Ct ± SD) |
| Sc (104 to 10 CFU/mL) |
Td (107 CFUs/mL) | 103 (28.057 ± 0.089) |
102 (30.992 ± 0.207) |
| Td (104 to 10 CFU/mL) |
Sc (107 CFUs/mL) | 103 (29.922 ± 0.387) |
102 (32.121 ± 0.185) |
| Lt (104 to 10 CFU/mL) |
Sc (107 CFUs/mL) | 103 (29.692 ± 0.280) |
102 (31.995 ± 0.759) |
| Mp (104 to 10 CFU/mL) |
Sc (107 CFUs/mL) | 104 (29.597 ± 0.495) |
103 (31.173 ± 1.799) |