. 2020 Sep 30;10(10):775. doi: 10.3390/diagnostics10100775

Table 1.

Oligonucleotide primers, probes, and blocker used in this study.

Assay Signature	Target Region	Primer Position	Primer/Probe	Sequence (5′ to 3′)	Concentration (Per Reaction)
PNA-mediated SARS-CoV-2 detection	Nucleocapsid	28371–28389 ^a	Forward primer	GCGATCAAAACAACGTCGG	250 nM
	Nucleocapsid	28575–28593 ^a	Reverse primer	ATACCATCTTGGACTGAGA	250 nM
	Nucleocapsid	28526–28545 ^a	Probe	FAM-ACCGAAGAGCTACCAGACGA-BHQ	250 nM
	Nucleocapsid	28486–28502 ^a	PNA blocker	ATCAACACCAATAGTGG ^c	500 nM
	Nucleocapsid	28342–28358 ^b	PNA blocker	ATCAACACCAATAGTGG ^c	500 nM
Universal SARSr-CoV detection	ORF3ab gene	26184–26201 ^a	Forward primer	CCGACGACGACTACTAGC	250 nM
	Envelope protein	26365–26386 ^a	Reverse primer	CTCACGTTAACAATATTGCAGC	250 nM
	Envelope protein	26329–26384 ^a	Probe	FAM-TAGCCATCCTTACTGCGCTT-BHQ	250 nM

^a Values were based on the Beta-CoV/Korea/KCDC03/2020 sequence. ^b Values were based on the SARS-CoV/HKU-39849 sequence. ^c The italic characters indicate the PNA blocker sequence.