Table 1.
Primer sequences, amplicon sizes, and amplification programs. F, forward; R, reverse.
| Gene | Primer Sequences | Amplicon Size (bp) | Amplification Program |
|---|---|---|---|
| nuc | F: TRGGCAGTAGGATTCGTTAA R: CTTTTGTGCTYCMTTTTGG |
926 | 94 °C 5 min; 94 °C 30 s, 58 °C 60 s, 72 °C 90 s, for 30 cycles; 72 °C 5 min. |
| hlb | F: GACGAAAATCAAGCGGAA R: TCTAAATACTCTGGCGCAC |
734 | 94 °C 2.5 min; 94 °C 30 s, 56 °C 30 s, 72 °C 1 min, for 30 cycles; 72 °C 10 min. |
| mecA | F: TCCACCCTCAAACAGGTGAA R: GGAACTTGTTGAGCAGAGGT |
139 | 94 °C 5 min; 94 °C 30 s, 55 °C 40 s, 72 °C 30 s, for 30 cycles; 72 °C 5 min. |