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. 2020 Oct 12;13(10):302. doi: 10.3390/ph13100302

Figure 2.

Figure 2

RAME decreases FOXM1 levels and the expression of its target genes by inhibiting the enrichment of FOXM1 on promoters. (A) Immunoblot analysis of FOXM1 (left) and its quantitative graph (right) in SKOV-3 cells. (B) Immunoblot analysis of FOXM1 (left) and its quantitative graph (right) in TOV-21G cells. (C,D) Chromatin Immunoprecipitation (ChIP)-qPCR analysis with FOXM1 (C) and H3Ac (D) antibody on CCNB1 and UBE2C promoters in RAME-treated SKOV-3 cells treated with 40 µM of RAME for 24 h. (E,F) Chromatin Immunoprecipitation (ChIP)-qPCR analysis with FOXM1 (E) and H3Ac (F) antibody on CCNB1 and UBE2C promoters in RAME-treated TOV-21G cells treated with 40 µM of RAME for 24 h. Error bars represent the mean ± SEM (n = 3). * p < 0.05, ** p < 0.01, *** p < 0.001; unpaired t-test.