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. 2020 Oct 10;13(10):300. doi: 10.3390/ph13100300

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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DNA transfection efficacy evaluation. The PANC-1 (a), HeLa (b) and 293T (c) cell lines were transfected with RGD1/DNA, RGD2/DNA, RGD3/DNA and RGD0/DNA polyplexes at charge ratios of 8/1 and 12/1, pCMV-lacZ plasmid alone and PEI/DNA. The transfection experiments were performed in the absence or presence of chloroquine. The PANC-1 cell line (d) was transfected with complexes of pEXPR-IBA5-eGFP plasmid and RGD1 and RGD0 carriers with charge ratios of 8/1 and 12/1. PEI polyplexes at an 8/1 charge ratio wereapplied as the control. Competitive transfection (e): the PANC-1 cells were transfected with RGD1/DNA and RGD0/DNA complexes at charge ratios of 8/1, pCMVlacZ plasmid alone and PEI/DNA. A free cyclo(RGDfK) ligand was added to the cells to compete with the complexes for binding of αvβ3 integrins. Reporter lacZ gene expression is given as milliunits (mU) per milligram of protein. GFP gene expression is given as a percentage of GFP-positive cells according to cytometry data. Values are the mean ± SD of the mean of triplicate experiments. * p < 0.05, ** p < 0.01 compared to RGD0/DNA complexes or compared to untreated complexes.

DNA transfection efficacy evaluation. The PANC-1 (a), HeLa (b) and 293T (c) cell lines were transfected with RGD1/DNA, RGD2/DNA, RGD3/DNA and RGD0/DNA polyplexes at charge ratios of 8/1 and 12/1, pCMV-lacZ plasmid alone and PEI/DNA. The transfection experiments were performed in the absence or presence of chloroquine. The PANC-1 cell line (d) was transfected with complexes of pEXPR-IBA5-eGFP plasmid and RGD1 and RGD0 carriers with charge ratios of 8/1 and 12/1. PEI polyplexes at an 8/1 charge ratio wereapplied as the control. Competitive transfection (e): the PANC-1 cells were transfected with RGD1/DNA and RGD0/DNA complexes at charge ratios of 8/1, pCMVlacZ plasmid alone and PEI/DNA. A free cyclo(RGDfK) ligand was added to the cells to compete with the complexes for binding of αvβ3 integrins. Reporter lacZ gene expression is given as milliunits (mU) per milligram of protein. GFP gene expression is given as a percentage of GFP-positive cells according to cytometry data. Values are the mean ± SD of the mean of triplicate experiments. * p < 0.05, ** p < 0.01 compared to RGD0/DNA complexes or compared to untreated complexes.