Skip to main content
. 2020 Sep 28;9(10):2187. doi: 10.3390/cells9102187

Figure 1.

Figure 1

Aquaporin-4 (AQP4) expression in the cuprizone model. (A1) Representative image demonstrating expression of AQP4 (red) in eGFP-expressing astrocytes (green). (A2) Relative anti-AQP4 staining intensities (control and 5 weeks cuprizone) within the area covered by single eGFP-expressing astrocytes. Data are shown as median + IQR; tested via Mann-Whitney U; co n = 44, cup n= 39. (B) AQP4 expression in control (co) animals. The lateral corpus callosum (b’), medial corpus callosum (b’’), and grey matter cortex (b’’’) are shown in higher magnification. The star highlights the hippocampal fimbria region; the arrow highlights the habenula; the arrowheads highlight AQP4-immunoreactive capillaries. (C) AQP4 expression in animals intoxicated with cuprizone for 5 weeks. The lateral corpus callosum (c’), medial corpus callosum (c’’), and grey matter cortex (c’’’) are shown in higher magnification. The hash sign highlights the hippocampal alveus. Scales: (B,C) 180 µm, (b’–b’’’,c’–c’’’) 90 µm, inserts 30 µm. (D) Semi-automatic optical density analyses of anti-AQP4 immunoreactivity in the lateral (latCC) and medial (medCC) corpus callosum in control mice and after 5 weeks cuprizone intoxication. (E) Semi-automatic quantification of anti-AQP4-positive microvessel densities in the cortex (Cx) per mm² in control mice and after 5 weeks cuprizone intoxication (top: absolute values, bottom: relative values). Data are shown as median + IQR; tested via repeated measures ANOVA; n = 3 per group; * = p < 0.05, ** = p < 0.01.