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Protection conferred by live attenuated IHNV vaccine in trout. Survival curves (a) and total mortality (b) of trout vaccinated with live attenuated IHNV intranasally (IN), by intramuscular (i.m.) injection or by immersion (imm). Trout (N = 25 per tank in duplicate tanks) were challenged to virulent IHNV i.p. 7 dpv (5 PFU). Unchal, unchallenged; Chal1 and Chal2 refer to duplicate tanks that were challenged for each experimental group.
